Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics

Storing rumen fluid (RF) has the potential to standardize subsequent in vitro feed fermentation studies. The first phase of this experiment aimed evaluate effect two RF storage methods on gas composition and dry matter disappearance (DMD) wheat grain lucerne hay under fermentation. were as follows: (1) snap-freezing using liquid nitrogen then storing it at −80 °C (−80 °C); (2) mixing with 5% dimethyl sulfoxide (DMSO), subsequently freezing −20 (D−20 °C), comparing fresh days 1, 14, 30 post collection. objective second was quantify impact preserving for 180 D−20 parameters. methane lower (p < 0.001) both preserved RFs than RF. There no difference 0.05) DMD values between 14 30. average cumulative production from stored higher that °C. Moreover, there day total lag time when fermenting Therefore, is preferable access limited.